Seasonal dynamics and genomic characteristics of ICU derived drug-resistant pathogens: significance of phage therapy
Background: The infection of drug-resistant pathogens in intensive care units (ICUs) is a major global public health challenge, with a mortality rate as high as 40-60%. Carbapenem resistant Klebsiella pneumoniae and Acinetobacter baumannii are the main pathogens causing ICU infections, and the seasonal dynamic transmission of resistance genes is closely related to environmental factors, medical procedures, and pathogen adaptability. The aim of this study is to reveal the seasonal distribution characteristics and genomic mechanisms of ICU resistant pathogens, providing a basis for precise prevention and control.
resistance genes: the blaCTX-M-3 gene carrier rate in autumn is as high as 100%, which is significantly correlated with increased bed turnover (59.4%) and prolonged disinfection intervals (OR=2.31); During winter, 75% of the clones were positive for qacE Δ 1, and their resistance index (0.82 ± 0.08) was significantly higher than that of the negative strains (0.3 ± 0.2). They also adapted to low temperature environments through disinfectant resistance and biofilm formation. In addition, the prevalence of aminoglycoside resistance genes (aac (6 ') - Ip/Ab7) was the highest (57%), indicating that there is a high pressure on the use of such antibiotics in the ICU.